IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00563912" target="_blank" >RIV/61389030:_____/22:00563912 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00126911 RIV/61989592:15310/22:73615537
Result on the web
<a href="https://doi.org/10.3389/fpls.2022.932008" target="_blank" >https://doi.org/10.3389/fpls.2022.932008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2022.932008" target="_blank" >10.3389/fpls.2022.932008</a>
Alternative languages
Result language
angličtina
Original language name
IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth
Original language description
Cytokinin and auxin are plant hormones that coordinate many aspects of plant development. Their interactions in plant underground growth are well established, occurring at the levels of metabolism, signaling, and transport. Unlike many plant hormone classes, cytokinins are represented by more than one active molecule. Multiple mutant lines, blocking specific parts of cytokinin biosynthetic pathways, have enabled research in plants with deficiencies in specific cytokinin-types. While most of these mutants have confirmed the impeding effect of cytokinin on root growth, the ipt29 double mutant instead surprisingly exhibits reduced primary root length compared to the wild type. This mutant is impaired in cis-zeatin (cZ) production, a cytokinin-type that had been considered inactive in the past. Here we have further investigated the intriguing ipt29 root phenotype, opposite to known cytokinin functions, and the (bio)activity of cZ. Our data suggest that despite the ipt29 short-root phenotype, cZ application has a negative impact on primary root growth and can activate a cytokinin response in the stele. Grafting experiments revealed that the root phenotype of ipt29 depends mainly on local signaling which does not relate directly to cytokinin levels. Notably, ipt29 displayed increased auxin levels in the root tissue. Moreover, analyses of the differential contributions of ipt2 and ipt9 to the ipt29 short-root phenotype demonstrated that, despite its deficiency on cZ levels, ipt2 does not show any root phenotype or auxin homeostasis variation, while ipt9 mutants were indistinguishable from ipt29. We conclude that IPT9 functions may go beyond cZ biosynthesis, directly or indirectly, implicating effects on auxin homeostasis and therefore influencing plant growth.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
1664-462X
Volume of the periodical
13
Issue of the periodical within the volume
OCT 14
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
932008
UT code for WoS article
000877162300001
EID of the result in the Scopus database
2-s2.0-85140910283