SMC5/6 complex-mediated SUMOylation stimulates DNA-protein cross-link repair in Arabidopsis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00573057" target="_blank" >RIV/61389030:_____/23:00573057 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00131345 RIV/61989592:15310/23:73619449
Result on the web
<a href="https://doi.org/10.1093/plcell/koad020" target="_blank" >https://doi.org/10.1093/plcell/koad020</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plcell/koad020" target="_blank" >10.1093/plcell/koad020</a>
Alternative languages
Result language
angličtina
Original language name
SMC5/6 complex-mediated SUMOylation stimulates DNA-protein cross-link repair in Arabidopsis
Original language description
DNA-protein cross-links (DPCs) are highly toxic DNA lesions consisting of proteins covalently attached to chromosomal DNA. Unrepaired DPCs physically block DNA replication and transcription. Three DPC repair pathways have been identified in Arabidopsis (Arabidopsis thaliana) to date: the endonucleolytic cleavage of DNA by the structure-specific endonuclease MUS81, proteolytic degradation of the crosslinked protein by the metalloprotease WSS1A, and cleavage of the cross-link phosphodiester bonds by the tyrosyl phosphodiesterases TDP1 and TDP2. Here we describe the evolutionary conserved STRUCTURAL MAINTENANCE OF CHROMOSOMEs SMC5/6 complex as a crucial component involved in DPC repair. We identified multiple alleles of the SMC5/6 complex core subunit gene SMC6B via a forward-directed genetic screen designed to identify the factors involved in the repair of DPCs induced by the cytidine analog zebularine. We monitored plant growth and cell death in response to DPC-inducing chemicals, which revealed that the SMC5/6 complex is essential for the repair of several types of DPCs. Genetic interaction and sensitivity assays showed that the SMC5/6 complex works in parallel to the endonucleolytic and proteolytic pathways. The repair of zebularine-induced DPCs was associated with SMC5/6-dependent SUMOylation of the damage sites. Thus, we present the SMC5/6 complex as an important factor in plant DPC repair.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell
ISSN
1040-4651
e-ISSN
1532-298X
Volume of the periodical
35
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
1532-1547
UT code for WoS article
000933836600001
EID of the result in the Scopus database
2-s2.0-85153414772