An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575377" target="_blank" >RIV/61389030:_____/23:00575377 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s41588-023-01401-2" target="_blank" >https://doi.org/10.1038/s41588-023-01401-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41588-023-01401-2" target="_blank" >10.1038/s41588-023-01401-2</a>

Result language
angličtina
Original language name
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
Original language description
The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with disease-resistance genes 1. Here we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata 2. We established that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Aegilops triuncialis 3, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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