Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575767" target="_blank" >RIV/61389030:_____/23:00575767 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/23:00130612 RIV/00216208:11310/23:10467497
Result on the web
<a href="https://doi.org/10.1007/s00497-023-00471-w" target="_blank" >https://doi.org/10.1007/s00497-023-00471-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00497-023-00471-w" target="_blank" >10.1007/s00497-023-00471-w</a>

Result language
angličtina
Original language name
Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
Original language description
Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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