Acute and sub-acute oral toxicity assessment of the methanol root extract of Olax subscorpioidea Oliv. (Olacaceae) in mice and rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00578664" target="_blank" >RIV/61389030:_____/23:00578664 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/23:73622975

Result on the web

<a href="https://doi.org/10.1016/j.sajb.2023.10.051" target="_blank" >https://doi.org/10.1016/j.sajb.2023.10.051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2023.10.051" target="_blank" >10.1016/j.sajb.2023.10.051</a>

Result language

angličtina

Original language name

Acute and sub-acute oral toxicity assessment of the methanol root extract of Olax subscorpioidea Oliv. (Olacaceae) in mice and rats

Original language description

Olax subscorpioidea Oliv. is an important medicinal plant included in African traditional anticancer medicines. Little information about its safety and toxicity appears in the literature. This study thus aimed at evaluating the safety of its methanol root extract orally administered in laboratory animals. Fourier Transform Infrared (FTIR) spectrophotometric analysis of O. subscorpioidea extract was conducted. For acute toxicity, methods within the Organization for Economic Co-operation and Development (OECD) guidelines were used, with slight modifications. A single female mouse was orally administered a dose of 2,000 mg/kg body weight of O. subscorpioidea methanol root extract and was monitored for at least 24 h for any signs of toxicity or death. An additional four mice (n = 4, 2/sex) were similarly treated. Another female mouse was orally administered 5,000 mg/kg body weight and observed for at least 24 h. Additional four mice were similarly treated. For sub-acute toxicity, the extract was administered daily to Wistar rats for 21 days at 250 mg/kg, 500 mg/kg, and 1,000 mg/kg. General behaviours changes, haematological, biochemical, and histological examinations on rats’ organs were performed. The FTIR spectrum showed no peaks in the 2220–2260 cm−1 region, suggesting that no cyanide groups were present in the extract. A single oral dose at both 2000 mg/kg and 5000 mg/kg did not induce any signs of toxicity or mortality, indicating an oral median lethal dose (LD50) >5000 mg/kg. When compared to the controls, dose-dependent changes were observed in the red blood cells, haemoglobin and haematocrit levels at 500 mg/kg and 1000 mg/kg (p<0.05). Histopathological examinations showed severe vascular congestion in the liver, and interstitial congestion and haemorrhage with moderate glomerular damage in the kidney. This study showed O. subscorpioidea root's methanol extract as non-toxic in acute administration, but with potential for toxicity in sub-acute oral administration.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

South African Journal of Botany

ISSN

0254-6299

e-ISSN

1727-9321

Volume of the periodical

163

Issue of the periodical within the volume

DEC

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

157-164

UT code for WoS article

001110294300001

EID of the result in the Scopus database

2-s2.0-85174951185