Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00579344" target="_blank" >RIV/61389030:_____/23:00579344 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/23:73622847
Result on the web
<a href="https://doi.org/10.1111/tpj.16456" target="_blank" >https://doi.org/10.1111/tpj.16456</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/tpj.16456" target="_blank" >10.1111/tpj.16456</a>
Alternative languages
Result language
angličtina
Original language name
Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution
Original language description
Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA20-22875S" target="_blank" >GA20-22875S: Organelle-specific gene expression and hormone dynamics during heat stress and high light responses</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Journal
ISSN
0960-7412
e-ISSN
1365-313X
Volume of the periodical
116
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
1825-1841
UT code for WoS article
001070054000001
EID of the result in the Scopus database
2-s2.0-85170546885