Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00585625" target="_blank" >RIV/61389030:_____/24:00585625 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/24:73622044
Result on the web
<a href="https://doi.org/10.1111/ppl.14144" target="_blank" >https://doi.org/10.1111/ppl.14144</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.14144" target="_blank" >10.1111/ppl.14144</a>
Alternative languages
Result language
angličtina
Original language name
Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state
Original language description
Light is an essential environmental signal for plant development called photomorphogenesis. Here, we show that diethyl ether anaesthesia inhibits the de-etiolation process in barley (Hordeum vulgare) seedlings. Illuminated seedlings exposed to diethyl ether accumulated significantly less chlorophylls and chlorophyll-binding proteins, and exhibited reduced maximum quantum yield of photosystem II photochemistry (Fv/Fm). Although the direct effect of light necessary for the greening process, i.e. for the photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) catalysed by light-dependent protochlorophyllide oxidoreductase A (PORA), was not inhibited, the RNA-seq and qPCR analyses showed that light-induced expression of photosynthesis-associated nuclear genes (PhANGs) and genes encoding enzymes for chlorophyll biosynthesis were attenuated. On the other hand, transcription of chloroplast-encoded genes was not negatively affected by diethyl ether treatment during greening. Among the genes negatively regulated by light, PORA and PHYA were only slightly affected by diethyl ether. The effect of diethyl ether was fully reversible and, after its removal, the greening process was fully restored. Our data indicate that diethyl ether had two effects on greening: i) it inhibited the expression of PhANGs and chlorophyll biosynthesis-related genes irrespective of light conditions, ii) it blocked the light-induced expression of these genes and greening process of etiolated seedlings. Our study indicates that diethyl ether affects plastid biogenesis, which alters the orchestration of negative and positive regulators affecting phytochrome and/or retrograde signalling and does not allow expression of PhANGs. Thus, the plants are locked in an intermediate skoto-photomorphogenetic state in the light.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA21-03593S" target="_blank" >GA21-03593S: Anaesthesia in plants: signalling and responses</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
1399-3054
Volume of the periodical
176
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
e14144
UT code for WoS article
001142148500001
EID of the result in the Scopus database
2-s2.0-85182481312