Comprehensive LC-MS/MS analysis of nitrogen-related plant metabolites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00599605" target="_blank" >RIV/61389030:_____/24:00599605 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/24:73625135 RIV/00027006:_____/24:10177621
Result on the web
<a href="https://doi.org/10.1093/jxb/erae129" target="_blank" >https://doi.org/10.1093/jxb/erae129</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erae129" target="_blank" >10.1093/jxb/erae129</a>
Alternative languages
Result language
angličtina
Original language name
Comprehensive LC-MS/MS analysis of nitrogen-related plant metabolites
Original language description
We have developed and validated a novel LC-MS/MS method for simultaneously analyzing amino acids, biogenic amines, and their acetylated and methylated derivatives in plants. This method involves a one-step extraction of 2-5 mg of lyophilized plant material followed by fractionation of different biogenic amine forms, and exploits an efficient combination of hydrophilic interaction liquid chromatography (HILIC), reversed phase (RP) chromatography with pre-column derivatization, and tandem mass spectrometry (MS). This approach enables high-throughput processing of plant samples, significantly reducing the time needed for analysis and its cost. We also present a new synthetic route for deuterium-labeled polyamines. The LC-MS/MS method was rigorously validated by quantifying levels of nitrogen-related metabolites in seedlings of seven plant species, including Arabidopsis, maize, and barley, all of which are commonly used model organisms in plant science research. Our results revealed substantial variations in the abundance of these metabolites between species, developmental stages, and growth conditions, particularly for the acetylated and methylated derivatives and the various polyamine fractions. However, the biological relevance of these plant metabolites is currently unclear. Overall, this work contributes significantly to plant science by providing a powerful analytical tool and setting the stage for future investigations into the functions of these nitrogen-related metabolites in plants.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: TowArds Next GENeration Crops (TANGENC)</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
1460-2431
Volume of the periodical
75
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
5390-5411
UT code for WoS article
001209758200001
EID of the result in the Scopus database
2-s2.0-85202972721