Insight into chromatin compaction and spatial organization in rice interphase nuclei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00599781" target="_blank" >RIV/61389030:_____/24:00599781 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3389/fpls.2024.1358760" target="_blank" >https://doi.org/10.3389/fpls.2024.1358760</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2024.1358760" target="_blank" >10.3389/fpls.2024.1358760</a>
Alternative languages
Result language
angličtina
Original language name
Insight into chromatin compaction and spatial organization in rice interphase nuclei
Original language description
Chromatin organization and its interactions are essential for biological processes, such as DNA repair, transcription, and DNA replication. Detailed cytogenetics data on chromatin conformation, and the arrangement and mutual positioning of chromosome territories in interphase nuclei are still widely missing in plants. In this study, level of chromatin condensation in interphase nuclei of rice (Oryza sativa) and the distribution of chromosome territories (CTs) were analyzed. Super-resolution, stimulated emission depletion (STED) microscopy showed different levels of chromatin condensation in leaf and root interphase nuclei. 3D immuno-FISH experiments with painting probes specific to chromosomes 9 and 2 were conducted to investigate their spatial distribution in root and leaf nuclei. Six different configurations of chromosome territories, including their complete association, weak association, and complete separation, were observed in root meristematic nuclei, and four configurations were observed in leaf nuclei. The volume of CTs and frequency of their association varied between the tissue types. The frequency of association of CTs specific to chromosome 9, containing NOR region, is also affected by the activity of the 45S rDNA locus. Our data suggested that the arrangement of chromosomes in the nucleus is connected with the position and the size of the nucleolus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
1664-462X
Volume of the periodical
15
Issue of the periodical within the volume
MAY 28
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
1358760
UT code for WoS article
001242916700001
EID of the result in the Scopus database
2-s2.0-85195534718