The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00619087" target="_blank" >RIV/61389030:_____/25:00619087 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.crmeth.2025.101008" target="_blank" >https://doi.org/10.1016/j.crmeth.2025.101008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.crmeth.2025.101008" target="_blank" >10.1016/j.crmeth.2025.101008</a>
Alternative languages
Result language
angličtina
Original language name
The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research
Original language description
The development of canine immunotolerant monoclonal antibodies can accelerate the invention of new medicines for both canine and human diseases. We develop a methodology to clone the naive, somatically mutated variable domain repertoire from canine B cell mRNA using 50RACE PCR. A set of degenerate primers were then designed and used to clone variable domain genes into archival holding plasmid libraries. These archived variable domain genes were then combinatorially ligated to produce a scFv M13 phage library. Next- generation long-read and short-read DNA sequencing methodologies were developed to annotate features of the cloned library including CDR diversity and IGHV/IGKV/IGLV subfamily distribution. A synthetic immunoglobulin G was developed from this scFv library to the canine immune checkpoint receptor PD-1. This synthetic platform can be used to clone and annotate archived antibody variable domain genes for use in perpetuity in order to develop improved preclinical models for the treatment of complex human diseases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CELL REPORTS METHODS
ISSN
2667-2375
e-ISSN
2667-2375
Volume of the periodical
5
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
101008
UT code for WoS article
001456387300001
EID of the result in the Scopus database
2-s2.0-105000456065