The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00619171" target="_blank" >RIV/61389030:_____/25:00619171 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10497314
Result on the web
<a href="https://doi.org/10.1111/nph.70019" target="_blank" >https://doi.org/10.1111/nph.70019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.70019" target="_blank" >10.1111/nph.70019</a>
Alternative languages
Result language
angličtina
Original language name
The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii
Original language description
Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii. Here, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events. The thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase. These findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
246
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
1066-1083
UT code for WoS article
001438711600001
EID of the result in the Scopus database
2-s2.0-105003245968