Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638664" target="_blank" >RIV/61389030:_____/25:00638664 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.plaphy.2025.110283" target="_blank" >https://doi.org/10.1016/j.plaphy.2025.110283</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2025.110283" target="_blank" >10.1016/j.plaphy.2025.110283</a>

Result language

angličtina

Original language name

Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

Original language description

It is assumed that the production of somatic embryos (SEs), which closely resemble fully mature zygotic embryos (ZEs), increases the chance of producing somatic plants equivalent to seedlings. Desiccation is crucial to reduce the water content of SEs and to initiate biochemical changes that support germination and conversion into plants. We investigated the biological, histological, biochemical and molecular changes in the SEs of hybrid larch Larix eurolepis exposed to desiccation at high relative humidity (RH, 98 %), reduced RH (59 %), or a combination of both. Desiccation at 98 % RH (D98) resulted in starch reduction, sucrose synthesis and a decrease in abscisic acid content without affecting germination and plant frequencies. In addition, proteomic results showed that D98 directed SE metabolism towards the acquisition of desiccation tolerance and energy storage. These modifications were proved to be favorable for SE germination. Desiccation at 59 % RH (D59) reduced the water content to a level corresponding to that of dried zygotic embryos. It could negatively impact the germination rate. The response of D59 SEs was directed towards the activation of defense mechanisms, while modifications favoring desiccation tolerance were suppressed. The treatment, which combined the two desiccation modes successively, yielded the positive effects of D98 while reducing the water content of SEs, similar to D59. Overall, our results provide new insights into the processes associated with the desiccation of hybrid larch SEs. We have developed an effective desiccation method resulting in a reduced water content comparable to that of dry ZE, while ensuring a high germination rate and successful conversion into plants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

1873-2690

Volume of the periodical

229

Issue of the periodical within the volume

Dec

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

110283

UT code for WoS article

001612417000004

EID of the result in the Scopus database

2-s2.0-105012127153