GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638827" target="_blank" >RIV/61389030:_____/25:00638827 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/25:10178146

Result on the web

<a href="https://doi.org/10.32615/bp.2025.005" target="_blank" >https://doi.org/10.32615/bp.2025.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.32615/bp.2025.005" target="_blank" >10.32615/bp.2025.005</a>

Result language

angličtina

Original language name

GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing

Original language description

Virus-induced gene silencing (VIGS) using self-replicating viral vectors is a powerful technique for analysing plant genes and proteins. Conversely, analysing gene silencing provides insights into the infectivity, replication, movement and stability of viral vectors. This study investigates the VIGS of phytoene desaturase gene (PDS) in different Nicotiana species using a vector based on apple latent spherical virus (ALSV, species Cheravirus mali, ICTV 2023). The ALSV genome consists of RNA1 and RNA2, which were cloned separately into plasmids using the GoldenBraid 3.0 system and transformed into two Agrobacterium strains. A third plasmid containing p19, a known gene silencing suppressor, was also introduced. These threeAgrobacterium cultures are traditionally grown separately and then inoculated together (as a mixture) in plants. Here, we describe a novel All-in-One system in which RNA1, RNA2 and p19 are co-expressed in plants after inoculation of a single Agrobacterium strain. A RT-qPCR analysis comparing both methods revealed that PDS expression is significantly different in the early phase of inoculation (6 dpi), but becomes statistically similar at later time points. The All-in-One approach therefore enables efficient co-expression of multiple targets, minimises variations in gene expression and reduces handling complexity, space requirements and costs compared to conventional co-inoculation methods.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

1573-8264

Volume of the periodical

69

Issue of the periodical within the volume

Jun

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

38-48

UT code for WoS article

001537894600001

EID of the result in the Scopus database

2-s2.0-105011769560