The Phenotype of Physcomitrium patens SMC6 Mutant with Interrupted Hinge Interactions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00639812" target="_blank" >RIV/61389030:_____/25:00639812 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00144615
Result on the web
<a href="https://doi.org/10.3390/genes16091091" target="_blank" >https://doi.org/10.3390/genes16091091</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/genes16091091" target="_blank" >10.3390/genes16091091</a>
Alternative languages
Result language
angličtina
Original language name
The Phenotype of Physcomitrium patens SMC6 Mutant with Interrupted Hinge Interactions
Original language description
Background/Objectives: nThe Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss Physcomitrium patens (P. patens). nMethods: nUsing CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the hinge domain of SMC6 of P. patens and show that both mutations are not toxic and allow viability of mutant lines. nResults: nThe G514R mutation fully prevents the interaction of SMC6 not only with SMC5, but also with NSE5 and NSE6, while the mutation at G517R has no effect. The Ppsmc6_G514R line has aberrant morphology, spontaneous and bleomycin-induced mutagenesis, and maintenance of the number of rDNA copies. The most unique feature is the interference with gene targeting (GT), which is completely abolished. In contrast, the Ppsmc6_G517R line is close to WT in many aspects. Surprisingly, both mutations have no direct effect on the rate of DSB repair in dividing and differentiated cells. nConclusions: nAbolished interactions of SMC6 with SMC5 and NSE5,6 partners, which allow DSB repair, but impair other repair and recombination functions, suggests also regulatory role for SMC6.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genes
ISSN
2073-4425
e-ISSN
2073-4425
Volume of the periodical
16
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
1091
UT code for WoS article
001579980600001
EID of the result in the Scopus database
2-s2.0-105017393204