Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00640005" target="_blank" >RIV/61389030:_____/25:00640005 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/25:73634916
Result on the web
<a href="https://doi.org/10.1111/pce.70103" target="_blank" >https://doi.org/10.1111/pce.70103</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/pce.70103" target="_blank" >10.1111/pce.70103</a>
Alternative languages
Result language
angličtina
Original language name
Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures
Original language description
Temperature sensing to adjust developmental rates and phenological responses to different climatic environments is critical for plant survival. Population-based thermal-time threshold models predict linear relationships between temperature and, for example, seed germination rates (speed), but the mechanisms are not known. Here, we used an integrative approach into the molecular biomechanical mechanisms underpinning a thermal-time model by combining Lepidium sativum micropylar endosperm (CAP) and radicle transcriptome analysis at defined heat units (generated by different time-temperature combinations) with corresponding CAP biomechanics. The thermal-time model delivered linear relationships with germination rates, but the underpinning biomechanical mechanisms of CAP weakening differed fundamentally between the optimal (24 degrees C-27 degrees C), sub-optimal (colder: 11 degrees C, 18 degrees C) and supra-optimal (warmer: 32 degrees C) temperatures. Chilling (11 degrees C) differed from other temperatures in that its CAP weakening inhibition was combined with altered CAP stiffness/elasticity. Differentially expressed cell wall remodelling protein (CWRP) genes associated with CAP weakening and/or stiffness/elasticity were identified using defined heat unit comparisons. Xyloglucans, galactomannans, pectins and UDP-sugar metabolism were major targets. Temperature regulation of CAP CWRP expression by DELAY-OF-GERMINATION-1 (DOG1) controls CAP weakening and seed germination. We conclude that distinct and temperature-specific molecular and biomechanical mechanisms underpin the apparently linear thermal-time responses during CAP weakening and seed germination.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell and Environment
ISSN
0140-7791
e-ISSN
1365-3040
Volume of the periodical
48
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
21
Pages from-to
8047-8067
UT code for WoS article
001544209000001
EID of the result in the Scopus database
2-s2.0-105012410367