Differences in the Improvement of Regeneration Ability of Wheat Calli by Ascorbate and H2O2 Through Modulation of Their Hormone and Metabolite Profile
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00642306" target="_blank" >RIV/61389030:_____/25:00642306 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00344-025-11849-7" target="_blank" >https://doi.org/10.1007/s00344-025-11849-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00344-025-11849-7" target="_blank" >10.1007/s00344-025-11849-7</a>
Alternative languages
Result language
angličtina
Original language name
Differences in the Improvement of Regeneration Ability of Wheat Calli by Ascorbate and H2O2 Through Modulation of Their Hormone and Metabolite Profile
Original language description
Successful plant regeneration from calli is important during transformation and micropropagation of plants. In order to increase its efficiency, the redox environment of the wheat calli was modified. Calli were treated with 0, 10, 20 and 40 mM ascorbate (Asc) or H2O2 for one week to compare their influence on shoot regeneration and the redox and hormonal regulatory mechanisms. Both compounds increased shoot formation in 10 mM concentration which could be the consequence of the mild oxidative stress indicated by increased H2O2 levels. However, only 20 mM H2O2 improved regeneration, while 20 mM Asc and both compounds at 40 mM levels had no such effect. Asc in 20 mM concentration was already very harmful based on the low survival of calli which could be the result of much larger increase in the ratio of oxidised glutathione compared to 20 mM H2O2. This change could contribute to the great reduction in the level of oxo-indole-3-acetic acid glucose-ester, trans-zeatin-glucosides and in isopentenyladenosine and its 9-glucoside in the more oxidising redox environment after 20 mM Asc treatment. These redox and hormonal changes after 20 mM Asc addition might also lead to much greater decrease of polyamine levels compared to 20 mM H2O2. In 10 mM concentration, both compounds improved shoot regeneration through their influence on metabolism, but at 20 mM level only H2O2 exhibited such positive effect, and Asc was harmful. Thus, H2O2 seems to be more appropriate for the induction of shoot formation during plant transformation and micropropagation.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/TH80020004" target="_blank" >TH80020004: Semitransparent PV coatings for greenhouse application</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Plant Growth Regulation
ISSN
0721-7595
e-ISSN
1435-8107
Volume of the periodical
44
Issue of the periodical within the volume
11
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
6630-6646
UT code for WoS article
001555970100001
EID of the result in the Scopus database
2-s2.0-105013983651