Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00644589" target="_blank" >RIV/61389030:_____/25:00644589 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/25:10509657

Result on the web

<a href="https://doi.org/10.3389/fpls.2025.1694769" target="_blank" >https://doi.org/10.3389/fpls.2025.1694769</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2025.1694769" target="_blank" >10.3389/fpls.2025.1694769</a>

Result language

angličtina

Original language name

Exocyst subunits EXO70B1 and B2 contribute to stomatal dynamics and cell wall modifications

Original language description

Introduction:nBased on previous reports of defense-related defects in the Arabidopsis loss-of-function (LOF) mutants of the EXO70B1 and EXO70B2 exocyst subunits, we investigated the underlying causes of these phenotypes.nMethods:nWe analyzed stomatal aperture states in both young and adult plants. As the exocyst is involved in the secretion to the cell wall, we examined cell wall composition, and we correlated these findings via a comprehensive mRNA expression analysis.nResults:nOur results revealed and discovered a more closed initial state of stomatal opening in exo70B mutants, as well as altered methylation and acetylation modifications of pectin and hemicellulose in the studied mutant lines. These changes in cell wall modifications may contribute to both compromised stomatal aperture-dependent and stomatal aperture-independent defense responses, as well as to the transcriptional activation of defense pathways observed in non-infected mutant plants at adult developmental stages. Several candidate genes involved in these processes were pinpointed using RNA-seq analysis.nDiscussion:nInterestingly, although the primary phenotypic and RNA-seq deviations in young mutant lines may be specific for each of the two EXO70B mutant lines, they exhibit converging gene expression profiles in later developmental stages. This convergence may reflect the shared evolutionary origin of the two Brassicaceae EXO70 isoforms by duplication from a common ancestral gene.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Volume of the periodical

16

Issue of the periodical within the volume

DEC 17

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

1694769

UT code for WoS article

001651464700001

EID of the result in the Scopus database

2-s2.0-105026762856