Label-Free Rapid Quantification of Abscisic Acid in Xylem Sap Samples Using Surface Plasmon Resonance
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00645085" target="_blank" >RIV/61389030:_____/25:00645085 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/25:73634902
Result on the web
<a href="https://doi.org/10.3390/bios15110725" target="_blank" >https://doi.org/10.3390/bios15110725</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/bios15110725" target="_blank" >10.3390/bios15110725</a>
Alternative languages
Result language
angličtina
Original language name
Label-Free Rapid Quantification of Abscisic Acid in Xylem Sap Samples Using Surface Plasmon Resonance
Original language description
The phytohormone abscisic acid (ABA) plays a central role in organizing adaptive responses in plants to various abiotic stresses, helping the plant minimize the negative impact on growth and development. Rapid and direct detection of ABA is valuable for investigating plant responses to abiotic stress. In this work, we propose a novel label-free, non-competitive immunoassay for detecting and quantifying ABA easily and rapidly using a surface plasmon resonance (SPR) biosensor. The SPR sensor chip was functionalized with a commercial anti-ABA antibody, characterized for its affinity, binding kinetics, and specificity using the same platform. The direct assay demonstrated high specificity and sensitivity, with a calculated limit of detection of 1.36 ng/mL in buffer. The new immunosensor was applied to determine ABA concentrations directly in xylem sap samples from tomato plants subjected to abiotic stress (drought and high salinity) and was able to accurately reflect ABA levels corresponding to the applied stress. The results were comparable to the reference method, ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), establishing this new immunosensor as a novel detection method for rapid and reliable monitoring of ABA levels associated with abiotic stress in tomato plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors
ISSN
2079-6374
e-ISSN
2079-6374
Volume of the periodical
15
Issue of the periodical within the volume
11
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
725
UT code for WoS article
001624063900001
EID of the result in the Scopus database
2-s2.0-105022975558