Meta-Topolin as an Aromatic Cytokinin for In Vitro Propagation of Thymus vulgaris L.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00645459" target="_blank" >RIV/61389030:_____/25:00645459 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/25:73634824
Result on the web
<a href="https://doi.org/10.3390/plants14233567" target="_blank" >https://doi.org/10.3390/plants14233567</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants14233567" target="_blank" >10.3390/plants14233567</a>
Alternative languages
Result language
angličtina
Original language name
Meta-Topolin as an Aromatic Cytokinin for In Vitro Propagation of Thymus vulgaris L.
Original language description
Effective in vitro propagation of medicinal and aromatic plants such as Thymus vulgaris is often limited by the choice of optimal cytokinin sources, which are critical for successful shoot proliferation and overall regeneration. Since their discovery, topolins have been recognized as alternatives to traditional cytokinins (CKs) such as benzyladenine (BA) and kinetin (Kin) in plant tissue culture (PTC). This study investigated the influence of three aromatic CKs (meta-topolin (mT), BA, and Kin), on shoot proliferation of thyme, with the goal of improving current PTC protocols. Of all the tested treatments, the highest shoot proliferation (7.25 +/- 0.72 shoots per nodal explant) was observed in the treatment with 1 mu M mT, superior to BA and Kin. Increasing mT and BA concentrations from 1 to 15 mu M had an inverse effect on shoot production. The addition of indole-3-butyric acid (IBA) at concentrations ranging from 1.0 to 5.0 mu M in combination with 0.5 and 1 mu M mT did not increase the mean shoot numbers per explant. Regenerated shoots exhibited a strong propensity for root development even in the absence of plant growth regulators with 100% survival ex vitro. This study demonstrated that mT is an effective, sustainable and cost-effective alternative to traditional CKs for in vitro propagation of T. vulgaris, achieving an improved shoot proliferation with 1 mu M mT application.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants
ISSN
2223-7747
e-ISSN
2223-7747
Volume of the periodical
14
Issue of the periodical within the volume
23
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
3567
UT code for WoS article
001634342700001
EID of the result in the Scopus database
2-s2.0-105024596376