OSMAC strategy and LC-MS profiling reveal metabolic diversity and bioactivities of Xylaria thienhirunae SWUF17-44.1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F26%3A00647285" target="_blank" >RIV/61389030:_____/26:00647285 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1186/s13213-025-01838-2" target="_blank" >https://doi.org/10.1186/s13213-025-01838-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13213-025-01838-2" target="_blank" >10.1186/s13213-025-01838-2</a>

Result language

angličtina

Original language name

OSMAC strategy and LC-MS profiling reveal metabolic diversity and bioactivities of Xylaria thienhirunae SWUF17-44.1

Original language description

Background Xylariaceous fungi represent a promising source of secondary metabolites with diverse biological functions. However, their metabolic capacity often remains underestimated under conventional cultivation. The One Strain-Many Compounds (OSMAC) strategy enhances metabolite diversity by altering culture conditions. This study investigated the metabolic potential and bioactivities of Xylaria thienhirunae SWUF17-44.1 using the OSMAC approach. Methods The strain was cultured under four conditions: Czapek yeast extract agar (CYA), Modified Norkrans's C (MNC), potato dextrose broth (PDB), and soluble malt yeast extract agar (SMYA). Extracts were evaluated for antimicrobial activity against bacteria and fungi, antioxidant capacity by DPPH radical scavenging and total phenolic content (TPC), and anti-inflammatory activity via nitric oxide inhibition. Metabolite profiling was performed by thin-layer chromatography and liquid chromatography-mass spectrometry (LC-MS). Results Culture media markedly influenced growth, metabolite yield, and bioactivity. Crude extracts displayed broad-spectrum antimicrobial properties, with stronger activity against Gram-positive bacteria. Antioxidant activity was higher in PDB, whereas TPC was higher in the nutrient-deficient media (CYA and SMYA).This indicates that compounds contributing to TPC do not necessarily drive antioxidant activity. The anti-inflammatory activity was highest in SMYA extracts. LC-MS analysis revealed common and unique metabolites across media, including alkaloids, aminoglycosides, fatty acid derivatives, and phenolics. Identified compounds included p-acetamidophenol, di-homo gamma-linolenoyl-EA, kolanone, and netilmicin. Several unassigned peaks indicated the presence of potentially novel metabolites. Conclusion The OSMAC strategy successfully enhanced the metabolic diversity of X. thienhirunae SWUF17-44.1, leading to extracts with antimicrobial, antioxidant, and anti-inflammatory properties. These findings highlight the strain as a promising source of bioactive compounds and underscore the utility of OSMAC in fungal natural product discovery.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA23-05389S" target="_blank" >GA23-05389S: Novel CB2 and BChE modulators against Parkinson's disease and related pathologies</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Microbiology

ISSN

1590-4261

e-ISSN

1869-2044

Volume of the periodical

76

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

9

UT code for WoS article

001674400300001

EID of the result in the Scopus database

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