Sorghum embryos undergoing B chromosome elimination express B-variants of mitotic-related genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F26%3A00647381" target="_blank" >RIV/61389030:_____/26:00647381 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1186/s13059-025-03915-w" target="_blank" >https://doi.org/10.1186/s13059-025-03915-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13059-025-03915-w" target="_blank" >10.1186/s13059-025-03915-w</a>

Result language

angličtina

Original language name

Sorghum embryos undergoing B chromosome elimination express B-variants of mitotic-related genes

Original language description

Background: Selective DNA elimination occurs across diverse species and plays a crucial role in evolution and development. This process encompasses small deletions, complete removal of chromosomes, or even the elimination of entire parental genomes. Despite its importance, the molecular mechanisms governing selective DNA elimination remain poorly understood. Our study focuses on the tissue-specific elimination of Sorghum purpureosericeum B chromosomes during embryo development. Results: In situ B chromosome visualisation, complemented by transcriptomic profiling and gene-enrichment analysis, allows us to identify 28 candidate genes potentially linked to chromosome elimination. We show that elimination is a developmentally programmed process, peaking during mid-embryogenesis and nearly completed at later stages, leaving B chromosomes only in restricted meristematic regions. Genome sequencing reveals that the sorghum B chromosome is of multi-A chromosomal origin, has reduced gene density, is enriched in repetitive sequences, and carries a novel centromeric repeat, SpuCL166. Transcriptome analyses identify B-specific variants of kinetochore, cohesion, and checkpoint genes that are expressed during active elimination, while structural modeling of CENH3 and CENP-C indicates functional divergence at the kinetochore interface. Conclusions: Here, we provide the first comprehensive genomic and transcriptomic characterization of B chromosome and its elimination in Sorghum purpureosericeum. Our findings suggest that B chromosomes express modified mitotic machinery to control their own fate. By establishing a framework of candidate genes, this study opens new avenues for dissecting the molecular mechanisms of chromosome elimination and provides a critical foundation for understanding how genomes evolve to regulate and tolerate supernumerary chromosomal elements.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2026

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome Biology

ISSN

1474-760X

e-ISSN

1474-760X

Volume of the periodical

27

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

27

Pages from-to

8

UT code for WoS article

001672474200001

EID of the result in the Scopus database

2-s2.0-105028728329