Expression and release of glucose-regulated protein-78 (GRP78) in multiple myeloma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F17%3AA1801TQZ" target="_blank" >RIV/61988987:17110/17:A1801TQZ - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/17:E0106546
Result on the web
<a href="http://dx.doi.org/10.18632/oncotarget.17353" target="_blank" >http://dx.doi.org/10.18632/oncotarget.17353</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.18632/oncotarget.17353" target="_blank" >10.18632/oncotarget.17353</a>
Alternative languages
Result language
angličtina
Original language name
Expression and release of glucose-regulated protein-78 (GRP78) in multiple myeloma
Original language description
Introduction: Multiple myeloma (MM) is a plasma cell neoplasm that is mostly incurable due to acquired resistance during the treatment course. Thus, we evaluated expression and release of glucose-regulated protein 78 kDa (GRP78/BiP), an endoplasmic reticulum (ER) based pro-survival chaperone involved in immunoglobulin folding and unfolded protein responses.Results: GRP78 protein expression in the ER and on the cell surface did not significantly differ between MGUS, NDMM and RRMM patients although there was a trend to higher surface expression in RRMM. In bone marrow plasma, the amount of released GRP78 protein was not significantly increased between MGUS-, NDMM- and RRMM patients. MM cells of the three cell lines release GRP78 as full-length protein under apoptotic, but not under acidotic or ER-stress conditions. In necrosis, only proteolytic fragments of GRP78 were detected in supernatants of MM cells.Materials and Methods: GRP78 protein expression and plasma levels were quantified in bone marrow aspirates of patients with monoclonal gammopathy of undetermined significance (MGUS, n = 29), newly diagnosed MM (NDMM, n = 29) and with relapsed/ refractory MM (RRMM, n = 15) by immunohistochemistry and sandwich ELISA. The human MM cell lines U266, NCI-H929 and OPM-2 were used for functional GRP78 release-and processing studies after induction of acidosis, ER stress, apoptosis and necrosis.Conclusions: Ectopic expression of GRP78 on cell membrane or its release in the microenvironment is not a suitable marker to distinguish MGUS from NDMM and RRMM.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
—
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncotarget
ISSN
1949-2553
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
34
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
56243-56254
UT code for WoS article
000408135600040
EID of the result in the Scopus database
2-s2.0-85029055013