Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F18%3AA1901Z8S" target="_blank" >RIV/61988987:17110/18:A1901Z8S - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/18:00101321 RIV/65269705:_____/18:00070322 RIV/00843989:_____/18:E0107125

Result on the web

<a href="http://pps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=12&SID=D53mK5Vm5TXNmRbzT3u&page=1&doc=5" target="_blank" >http://pps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=12&SID=D53mK5Vm5TXNmRbzT3u&page=1&doc=5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijmm.2018.04.008" target="_blank" >10.1016/j.ijmm.2018.04.008</a>

Result language

angličtina

Original language name

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Original language description

A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p &lt; 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p &lt; 0.01), including a higher prevalence of the colicin B determinant (p &lt; 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p &lt; 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p &lt; 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30219 - Gastroenterology and hepatology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Medical Microbiology

ISSN

1438-4221

e-ISSN

1618-0607

Volume of the periodical

308

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

498-504

UT code for WoS article

000440393900002

EID of the result in the Scopus database

2-s2.0-85046661589