Detection of monoclonal free light chains by immunofixation electrophoresis and isoelectric focusing - comparison with the quantitative method of determination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F20%3AA21026QC" target="_blank" >RIV/61988987:17110/20:A21026QC - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/20:E0108614
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/00365513.2020.1804608" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/00365513.2020.1804608</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00365513.2020.1804608" target="_blank" >10.1080/00365513.2020.1804608</a>
Alternative languages
Result language
angličtina
Original language name
Detection of monoclonal free light chains by immunofixation electrophoresis and isoelectric focusing - comparison with the quantitative method of determination
Original language description
The study aimed to investigate free light chain (FLC) monoclonality in patients with an abnormal free kappa/lambda ratio (FLC ratio). Seventy serum samples with abnormal FLC ratio were examined using an immunoturbidimetry (Binding Site, SPA) and the two different enzyme-linked immunosorbent assays (1. Sebia diagnostic kit; 2. in house methods), the monoclonal or oligoclonal bands of (FLC) by immunofixation electrophoresis (IE) and isoelectric focusing followed by affinity immunoblotting (IEF/AIB). The reference interval was calculated by non-parametric percentile method. 5.7% of samples examined by IE were suspected of monoclonal character of FLCs, but subsequently monoclonality was refuted by more sensitive IEF/AIB method; 7%, resp. 2.9% of samples showed FLC kappa, resp. FLC lambda oligoclonal character of bands. A statistically significant dependence was found between FLC ratio (Sebia) and FLC ratio (SPA), r(s)= 0.510,p = .001. Kappa statistic evaluated a fair conformity between the FLC ratio (Sebia) and IEF/AIB (kappa = 0.468) and between FLC ratio (in house) and IEF/AIB (kappa = 0.300).
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30502 - Other medical science
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
SCAND J CLIN LAB INV
ISSN
0036-5513
e-ISSN
1502-7686
Volume of the periodical
80
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
556-561
UT code for WoS article
000557956900001
EID of the result in the Scopus database
—