Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F18%3AA1901X11" target="_blank" >RIV/61988987:17310/18:A1901X11 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10376235

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-018-23575-0" target="_blank" >http://dx.doi.org/10.1038/s41598-018-23575-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-23575-0" target="_blank" >10.1038/s41598-018-23575-0</a>

Result language

angličtina

Original language name

Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase

Original language description

Rheb is a conserved and widespread Ras-like GTPase involved in cell growth regulation mediated by the (m) TORC1 kinase complex and implicated in tumourigenesis in humans. Rheb function depends on its association with membranes via prenylated C-terminus, a mechanism shared with many other eukaryotic GTPases. Strikingly, our analysis of a phylogenetically rich sample of Rheb sequences revealed that in multiple lineages this canonical and ancestral membrane attachment mode has been variously altered. The modifications include: (1) accretion to the N-terminus of two different phosphatidylinositol 3-phosphate-binding domains, PX in Cryptista (the fusion being the first proposed synapomorphy of this clade), and FYVE in Euglenozoa and the related undescribed flagellate SRT308; (2) acquisition of lipidic modifications of the N-terminal region, namely myristoylation and/or S-palmitoylation in seven different protist lineages; (3) acquisition of S-palmitoylation in the hypervariable C-terminal region of Rheb in apusomonads, convergently to some other Ras family proteins; (4) replacement of the C-terminal prenylation motif with four transmembrane segments in a novel Rheb paralog in the SAR clade; (5) loss of an evident C-terminal membrane attachment mechanism in Tremellomycetes and some Rheb paralogs of Euglenozoa. Rheb evolution is thus surprisingly dynamic and presents a spectacular example of molecular tinkering.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCIENTIFIC REPORTS

ISSN

2045-2322

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

březen

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000428366900001

EID of the result in the Scopus database

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