Determination of suitable insect part for non‐lethal DNA sampling: case study of DNA quality and regeneration capability of dragonflies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA21023M2" target="_blank" >RIV/61988987:17310/20:A21023M2 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/icad.12400" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/icad.12400</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/icad.12400" target="_blank" >10.1111/icad.12400</a>

Result language

angličtina

Original language name

Determination of suitable insect part for non‐lethal DNA sampling: case study of DNA quality and regeneration capability of dragonflies

Original language description

1. Genetics has been widely used in insect ecology and conservation. To minimize the effect of DNA sampling on organisms as much as possible, it would be ideal to use non-invasive or non-lethal DNA sources. Therefore, it is also very important to determine the responses of organisms to DNA sampling. 2. In this study, the quality and quantity of genomic DNA samples from three types of insect tissues (exuvia, mid-legs and wings) were evaluated. As model organisms, we used two dragonfly species of different sizes (Leucorrhinia dubia, Anax imperator). We also tested the regenerative ability of dragonfly larvae as a repair mechanism after mid-leg cut-off, with respect to factors such as size and quantity of diet. 3. We found that DNA of sufficient quality for analyses was obtained from all tested tissues. However, isolates from exuviae were conclusively less useful for sequencing than those from the mid-legs and wings. The highest quantity of DNA was obtained from the mid-legs. The survival of larvae is not affected by removing the legs, which can usually regenerate. 4. All the tested tissues could be a source of adequate DNA; however, we concluded that primarily the legs should be used because they provided the best DNA samples in terms of quantity and quality of DNA. Furthermore, their exploitation would not affect individuals seriously if young larvae with sufficient time (at least six months) for regeneration are sampled. The exuviae should be used for absolutely non-invasive studies involving endangered or protected species.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10616 - Entomology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INSECT CONSERV DIVER

ISSN

1752-458X

e-ISSN

1752-4598

Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

319-327

UT code for WoS article

000509329400001

EID of the result in the Scopus database

2-s2.0-85078665465