Cross-species amplification of microsatellites and identification of polyploid hybrids by allele dosage effects in Cobitis hankugensis x Iksookimia longicorpa hybrid complex (Actinopterygii: Cypriniformes: Cobitidae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202EE7" target="_blank" >RIV/61988987:17310/21:A2202EE7 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/21:00545135
Result on the web
<a href="https://aiep.pensoft.net/article/63591/" target="_blank" >https://aiep.pensoft.net/article/63591/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3897/aiep.51.63591" target="_blank" >10.3897/aiep.51.63591</a>
Alternative languages
Result language
angličtina
Original language name
Cross-species amplification of microsatellites and identification of polyploid hybrids by allele dosage effects in Cobitis hankugensis x Iksookimia longicorpa hybrid complex (Actinopterygii: Cypriniformes: Cobitidae)
Original language description
During the course of evolution, numerous taxa abandoned canonical sex and reproduced asexually. Examination of the Cobitis hankugensis x Iksookimia longicorpa asexual complex already revealed important evolutionary discoveries tackling phenomena like interspecific hybridization, non-Mendelian inheritance, polyploidy, and asexuality. Yet, as in other similar cases, the investigation is hampered by the lack of easily accessible molecular tools for efficient differentiation among genomotypes. Here, we tested the cross-species amplification of 23 microsatellite markers derived from distantly related species and investigated the extent to which such markers can facilitate the genome identification in the non-model hybrid complex. We found that 21 out of 23 microsatellite markers were amplified in all genomotypes. Five of them could be used for easy diagnostics of parental species and their hybrids due to species-specific amplification profiles. We also noted that three markers, i.e., IC654 and IC783 derived from Cobitis choii Kim et Son, 1984 and Iko_TTA01 from Iksookimia koreensis (Kim, 1975), had dosage-sensitive amplification efficiencies of species-specific alleles. This could be further used for reliable differentiation of genome composition in polyploids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACTA ICHTHYOLOGICA ET PISCATORIA
ISSN
0137-1592
e-ISSN
1734-1515
Volume of the periodical
51
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
8
Pages from-to
167-174
UT code for WoS article
000683381300007
EID of the result in the Scopus database
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