Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F22%3AA2402NBT" target="_blank" >RIV/61988987:17310/22:A2402NBT - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/22:00567811 RIV/60076658:12310/22:43906095

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2001037022005177" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2001037022005177</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.csbj.2022.11.023" target="_blank" >10.1016/j.csbj.2022.11.023</a>

Result language

angličtina

Original language name

Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations

Original language description

The kinetoplastids are unicellular flagellates that derive their name from the 'kinetoplast', a region within their single mitochondrion harboring its organellar genome of high DNA content, called kinetoplast (k) DNA. Some protein products of this mitochondrial genome are encoded as cryptogenes; their transcripts require editing to generate an open reading frame. This happens through RNA editing, whereby small regulatory guide (g)RNAs direct the proper insertion and deletion of one or more uridines at each editing site within specific transcript regions. An accurate perspective of the kDNA expansion and evolution of their unique uridine insertion/deletion editing across kinetoplastids has been difficult to achieve. Here, we resolved the kDNA structure and editing patterns in the early-branching kinetoplastid Trypanoplasma borreli and compare them with those of the well-studied trypanosomatids. We find that its kDNA consists of circular molecules of about 42 kb that harbor the rRNA and protein-coding genes, and 17 different contigs of approximately 70 kb carrying an average of 23 putative gRNA loci per contig. These contigs may be linear molecules, as they contain repetitive termini. Our analysis uncovered a putative gRNA population with unique length and sequence parameters that is massive relative to the editing needs of this parasite. We validated or determined the sequence identity of four edited mRNAs, including one coding for ATP synthase 6 that was previously thought to be missing. We utilized computational methods to show that the T. borreli transcriptome includes a substantial number of transcripts with inconsistent editing patterns, apparently products of non-canonical editing. This species utilizes the most extensive uridine deletion compared to other studied kinetoplastids to enforce amino acid conservation of cryptogene products, although insertions still remain more frequent.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA22-01026S" target="_blank" >GA22-01026S: RNA editing in non-model flagellates</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL

ISSN

2001-0370

e-ISSN

—

Volume of the periodical

—

Issue of the periodical within the volume

2022

Country of publishing house

SE - SWEDEN

Number of pages

14

Pages from-to

6388-6402

UT code for WoS article

001043880900008

EID of the result in the Scopus database

2-s2.0-85142138566