Virion structure of Leishmania RNA virus 1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F22%3AN2302JHP" target="_blank" >RIV/61988987:17310/22:N2302JHP - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/22:00127386 RIV/61988987:17310/22:A2302JHP

Result on the web

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000928392900004" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000928392900004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.virol.2022.09.014" target="_blank" >10.1016/j.virol.2022.09.014</a>

Result language

angličtina

Original language name

Virion structure of Leishmania RNA virus 1

Original language description

The presence of Leishmania RNA virus 1 (LRV1) enables Leishmania protozoan parasites to cause more severe disease than the virus-free strains. The structure of LRV1 virus-like particles has been determined previously, however, the structure of the LRV1 virion has not been characterized. Here we used cryo-electron microscopy and single-particle reconstruction to determine the structures of the LRV1 virion and empty particle isolated from Leishmania guyanensis to resolutions of 4.0 A and 3.6 A, respectively. The capsid of LRV1 is built from sixty dimers of capsid proteins organized with icosahedral symmetry. RNA genomes of totiviruses are replicated inside the virions by RNA polymerases expressed as C-terminal extensions of a sub-population of capsid proteins. Most of the virions probably contain one or two copies of the RNA polymerase, however, the location of the polymerase domains in LRV1 capsid could not be identified, indicating that it varies among particles.Importance.Every year over 200 000 people contract leishmaniasis and more than five hundred people die of the disease. The mucocutaneous form of leishmaniasis produces lesions that can destroy the mucous membranes of the nose, mouth, and throat. Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are predisposed to cause aggravated symptoms in the mucocutaneous form of leishmaniasis. Here, we present the structure of the LRV1 virion determined using cryo-electron microscopy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virology

ISSN

0042-6822

e-ISSN

1089-862X

Volume of the periodical

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Issue of the periodical within the volume

prosinec

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

149-154

UT code for WoS article

000928392900004

EID of the result in the Scopus database

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