New foundations for the physical mechanism of variable chlorophyll a fluorescence. Quantum efficiency versus the light-adapted state of photosystem II
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F23%3AA2402L4I" target="_blank" >RIV/61988987:17310/23:A2402L4I - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jxb/article-abstract/74/18/5458/7220642?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/jxb/article-abstract/74/18/5458/7220642?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erad252" target="_blank" >10.1093/jxb/erad252</a>
Alternative languages
Result language
angličtina
Original language name
New foundations for the physical mechanism of variable chlorophyll a fluorescence. Quantum efficiency versus the light-adapted state of photosystem II
Original language description
Photosystem II (PSII) uses solar energy to oxidize water and delivers electrons to fix CO2. Although the atomic resolution structure and basic photophysical and photochemical functions of PSII are well understood, many important questions remain. The activity of PSII in vitro and in vivo is routinely monitored by recording chlorophyll-a fluorescence induction kinetics (ChlF). According to the ‘mainstream’ model, the rise from the minimum level (Fo) to the maximum (Fm) of ChlF of dark-adapted PSII reflects the closure of all functionally active reaction centers, and the Fv/Fm ratio is equated with the maximum photochemical quantum yield of PSII (Fv=Fm–Fo). However, this model has never been free of controversies. A range of recent experimental data confirmed that the first single-turnover saturating flash (STSF), generating closed state (PSIIC), produces F1<Fm; and uncovered rate-limiting steps – Δτ1/2 half-waiting times – in the multi-STSF induced F1-to-Fm increments, originating from the gradual formation of light-adapted charge-separated state (PSIIL) with significantly increased stability of charges compared to PSIIC elicited by a single STSF. All data show that the interpretation of ChlF must be laid on new grounds. Here we discuss the underlying physical mechanisms and the significance of structural/functional dynamics of PSII – as reflected by ChlF and variations of the novel parameter Δτ1/2.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA23-07744S" target="_blank" >GA23-07744S: Identification and characterization of structural entities associated with different lipid phases of plant thylakoid membranes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
1460-2431
Volume of the periodical
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Issue of the periodical within the volume
18
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
5458-5471
UT code for WoS article
001066729300001
EID of the result in the Scopus database
2-s2.0-85173036044