Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F24%3AA2502NXK" target="_blank" >RIV/61988987:17310/24:A2502NXK - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1381040/full" target="_blank" >https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1381040/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2024.1381040" target="_blank" >10.3389/fpls.2024.1381040</a>

Result language

angličtina

Original language name

Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus

Original language description

In our earlier works, we have shown that the rate-limiting steps, associated with the dark-to-light transition of Photosystem II (PSII), reflecting the photochemical activity and structural dynamics of the reaction center complex, depend largely on the lipidic environment of the protein matrix. Using chlorophyll-a fluorescence transients (ChlF) elicited by single-turnover saturating flashes, it was shown that the half-waiting time (Δτ1/2) between consecutive excitations, at which 50% of the fluorescence increment was reached, was considerably larger in isolated PSII complexes of Thermostichus (T.) vulcanus than in the native thylakoid membrane (TM). Further, it was shown that the addition of a TM lipid extract shortened Δτ1/2 of isolated PSII, indicating that at least a fraction of the ‘missing’ lipid molecules, replaced by detergent molecules, caused the elongation of Δτ1/2. Here, we performed systematic experiments to obtain information on the nature of TM lipids that are capable of decreasing Δτ1/2. Our data show that while all lipid species shorten Δτ1/2, the negatively charged lipid phosphatidylglycerol appears to be the most efficient species – suggesting its prominent role in determining the structural dynamics of PSII reaction center.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/GA23-07744S" target="_blank" >GA23-07744S: Identification and characterization of structural entities associated with different lipid phases of plant thylakoid membranes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

1664-462X

Volume of the periodical

—

Issue of the periodical within the volume

21 March 2024

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

1-11

UT code for WoS article

001196265200001

EID of the result in the Scopus database

2-s2.0-85189307385