To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27360%2F25%3A10259727" target="_blank" >RIV/61989100:27360/25:10259727 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/smsc.202500446?getft_integrator=clarivate&src=getftr&utm_source=clarivate" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/smsc.202500446?getft_integrator=clarivate&src=getftr&utm_source=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/smsc.202500446" target="_blank" >10.1002/smsc.202500446</a>
Alternative languages
Result language
angličtina
Original language name
To Dye or Not to Dye: Unraveling the Impact of Surface Chemistry on Cerium Oxide Nanoparticles-Cell Interactions
Original language description
Colloidally stable cerium oxide nanoparticles (CeNPs), known for mimicking multiple antioxidant enzymes, are promising nanozymes for therapeutic applications targeting oxidative stress and inflammation. Although fluorescent dye labeling facilitates nanoparticle imaging and tracking, its influence on physicochemical properties and biological interactions remains insufficiently understood. In this study, poly(acrylic acid)-coated CeNPs and their DiI-encapsulated counterparts are synthesized to evaluate the effects of dye functionalization on catalytic performance, cellular uptake, and intracellular fate. While overall redox cycling is retained, DiI@PAA-CeNPs show an 30% decrease in superoxide dismutase-like activity, whereas catalase-, peroxidase-, and oxidase-like activities remain largely preserved. Both formulations exhibit no cytotoxicity toward human osteoblasts. However, DiI labeling modifies surface chemistry and delays cellular uptake, particularly in the presence of serum proteins, as revealed by fluorescence microscopy. Multiscale imaging combining fluorescence microscopy, transmission electron microscopy, and focused ion beam-assisted lamella preparation reveals differences in nanoparticle internalization but similar intracellular fate. Focusing on poly(acrylic acid)-coated cerium oxide nanoparticles, DiI encapsulation can markedly alter CeNPs properties. This finding highlights the need to critically assess labeling effects in bio-nano studies. Integrating label-free techniques is crucial for accurate characterization and the rational design of nanozymes for diagnostic and therapeutic applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21000 - Nano-technology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004631" target="_blank" >EH22_008/0004631: Materials and technologies for sustainable development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Small Science
ISSN
2688-4046
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
nestránkováno
UT code for WoS article
001641626800001
EID of the result in the Scopus database
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