Apoptosis induced by N6-substituted derivatives of adenosine is related to intracellular accumulation of corresponding mononucleotides in HL-60 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A00001968" target="_blank" >RIV/61989592:15110/05:00001968 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Apoptosis induced by N6-substituted derivatives of adenosine is related to intracellular accumulation of corresponding mononucleotides in HL-60 cells

Original language description

The in vitro induction of apoptosis by N6-substituted derivatives of adenosine and adenine was investigated in HL-60 cells. Using reversed phase HPLC/MS analysis we demonstrated that both N6-substituted derivatives of adenosine and adenine are phosphorylated within cells to the monophosphate level. While N6-substituted derivatives of adenosine were phosphorylated by adenosine kinase and corresponding mononucleotides were produced in large quantities, N6-substituted derivatives of adenine were convertedinto the corresponding mononucleotides via the phosphoribosyl transferase pathway, which yielded 50-100 times lower amounts of the mononucleotides than the adenosine kinase pathway. Accordingly, N6-substituted derivatives of adenine were relatively inefficient inductors of apoptosis at the concentrations applied. Inhibitors of adenosine kinase that abrogated the formation of monophosphates from N6-substituted derivatives of adenosine completely prevented cells from going into apoptosis.

Czech name

Apoptoza indukovaná N6-substituovanými deriváty adenosinu je spojena s intracelulární akumulací korespondujících mononukleotidů u buněk HL-60

Czech description

In vitro indukce apoptozy N6-substitouvanými deriváty adenosinu a adeninu byla zkoumána u buněk linie HL-60.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NC6652" target="_blank" >NC6652: Pathophyziologie of Chronic Myelogenous Leukemia - Cellular and Mouse Model of the Disease</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

985-990

UT code for WoS article

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EID of the result in the Scopus database

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