Expression of CCX CKR in pulmonary sarcoidosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F06%3A00003119" target="_blank" >RIV/61989592:15110/06:00003119 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Expression of CCX CKR in pulmonary sarcoidosis
Original language description
Objective: CCX CKR is a decoy chemokine receptor that specifically binds the chemokines CCL19, CCL25 and CCL21. CCL19 was previously found to be upregulated in pulmonary sarcoidosis. We have, therefore, investigated CCX CKR expression in this inflammatory disease. Methods and results: CCX CKR mRNA was semiquantitated using RT-PCR in unseparated bronchoalveolar (BAL) cells from sarcoidosis patients (S, n=29) and healthy control subjects (C, n=9). CCX CKR transcripts were upregulated in patients (mean+/-SEM); S, 0.82+/-0.10; C, 0.44+/-0.04; p=0.01; this upregulation paralleled the disease course as assessed by the chest radiographic stage (p<0.02). Immunocytochemistry localised the CCX CKR protein to ciliated bronchial cells. Flow cytometric fluorescentligand uptake assay showed that these cells are able to internalize its ligand. Conclusion: These observations implicate CCX CKR in the modulation of the inflammatory response in sarcoidosis.
Czech name
Exprese CCX CKR u plicní sarkoidózy
Czech description
CCX CKR je vábící chemokinový receptor pro CCL19, CCL25 a CCL21 chemokin, které se významně podílí na imunitní reakci u plicní sarkoidózy. V této práci jsme se proto zaměřili na expresi a roli CCX CKR v zánětlivém procesu sarkoidózy. Semikvantitativní analýza pomocí RT-PCR odhalila zvýšenou expresi CCX CKR mRNA v bronchoalveolárních buňkách od pacientů se sarkoidózou (S, n=29; C, n=9)(S, 0,82+/-0,10 C, 0,44+/-0,04; p= 0.01), zejména se CXR-stádiem III ve srovnání s počátečním stádiem I (S-I, 0,72+/-0,10; S-II, 0,85+/-0,20; S-III, 1,04+/-0,20, p<0,02). Imunohistochemie ukázala přítomnost CCX CKR na řasinkových bronchiálních buňkách a průtoková cytometrie potvrdila schopnost internalizace tohoto receptoru.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Inflammation Research
ISSN
1023-3830
e-ISSN
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Volume of the periodical
2006
Issue of the periodical within the volume
55
Country of publishing house
CH - SWITZERLAND
Number of pages
5
Pages from-to
441-445
UT code for WoS article
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EID of the result in the Scopus database
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