Mechanism-based inhibitors of cytokinin oxidase/dehydrogenase attack FAD cofactor.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00007206" target="_blank" >RIV/61989592:15110/08:00007206 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/08:00322290 RIV/61989592:15310/08:00005306
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Mechanism-based inhibitors of cytokinin oxidase/dehydrogenase attack FAD cofactor.
Original language description
Cytokinin oxidases/dehydrogenases (CKOs) mediate catabolic regulation of cytokinin levels in plants. Several substrate analogs containing an unsaturated side chain were studied for their possible inhibitory effect on maize CKO (ZmCKO1) by use of variousbioanalytical methods. Two allenic derivatives, N(6)-(buta-2,3-dienyl)adenine (HA-8) and N(6)-(penta-2,3-dienyl)adenine (HA-1), were identified as strong mechanism-based inhibitors of the enzyme. Despite exhaustive dialysis, the enzyme remained inhibited. Conversely, substrate analogs with a triple bond in the side chain were much weaker inactivators. The crystal structures of recombinant ZmCKO1 complexed with HA-1 or HA-8 were solved to 1.95 A resolution. Together with Raman spectra of the inactivatedenzyme, it was revealed that reactive imine intermediates generated by oxidation of the allenic inhibitors covalently bind to the flavin adenine dinucleotide (FAD) cofactor. The binding occurs at the C4a atom of the isoalloxazine ring of
Czech name
Mechanism-based inhibitory cytokinin oxidas/dehydrogenas interagují s FAD kofaktorem
Czech description
Byla charakterizována inhibice kukuřičné cytokinin oxidasy těmito látkami: N(6)-(buta-2,3-dienyl)adenine (HA-8) a N(6)-(penta-2,3-dienyl)adenine (HA-1).

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/GP522%2F08%2FP113" target="_blank" >GP522/08/P113: The role of FAD cofactor of cytokinin oxidase/dehydrogenase in the mechanism of cytokinin breakdown</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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