Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00007418" target="_blank" >RIV/61989592:15110/08:00007418 - isvavai.cz</a>

Alternative codes found

RIV/00020711:_____/08:00002043

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase

Original language description

AIMS: To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS: Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase) the possibility of specific detection of various control E. coli strains was tested. It was found that four PCR fragments of the predicted size were observed only for E. coli strains, but not for relatives as close as Shigella sp. or other enterobacteria. Not surprisingly, this method enabled us to identify also E. coli strains which did not exhibit the beta-D-glucuronidase activity. Our multiplex PCR was also successfully used for identification of 95 environmental isolates of E. coli. CONCLUSIONS: The developed PCR-based method, in which four genes coding for lactose permease, cytochrome bd complex, beta-d-glucuronidase, and beta-d-galactosidase, serve as target DNA s

Czech name

Specifická detekce Escherichia coli izolované ze vzorků vody pomocí polymerázové řetězové reakce, jež amplifikuje čtyři cílové geny: komplex cytochromu bd, laktosovou permeasu, beta-D-glukuronidasu, a beta-D-galaktosidasu

Czech description

Cílem práce bylo vyvinout metodu na bázi polymerázové řetězové reakce (PCR), jež umožňuje spolehlivou detekci Escherichia coli tak, aby ji bylo možno rozlišit od biochemicky a fylogeneticky příbuzných bakterií.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

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Volume of the periodical

105

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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