HIV-1 gp120 glycosylation is cell type-specific and influences reactivity with HIV-1-specific antibodies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213116" target="_blank" >RIV/61989592:15110/10:10213116 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
HIV-1 gp120 glycosylation is cell type-specific and influences reactivity with HIV-1-specific antibodies
Original language description
N-glycans of HIV-1 gp120 are thought to affect folding and shield the virus from reactivity with antibodies (Abs). We analyzed glycosylation patterns of gp120 produced in five different human and animal cell lines. Gp120 produced by human embryonic kidney and T cells contained mostly high-mannose glycans, whereas gp120 from human rhabdomyosarcoma and hepatocellular carcinoma and Chinese-hamster ovary cells displayed higher proportion of complex glycans. This differential glycosylation affected reactivities with V3-loop-specific monoclonal Abs (mAbs), as well as with Abs from sera of HIV-1-infected patients. Our results underscore the importance of selection of the cells producing recombinant glycoprotein(s) for immunization.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EC - Immunology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Centennial Retrovirus Meeting
ISBN
978-88-7587-586-2
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
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Publisher name
Medimond S.r.l. - Monduzzi Editore
Place of publication
Bologna
Event location
Praha
Event date
Apr 29, 2010
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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