Flow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10215584" target="_blank" >RIV/61989592:15110/10:10215584 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Flow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma
Original language description
The aim of this study was to develop, validate and compare FIA- and UHPLC-MS/MS methods for the determination of imatinib (IM) in plasma from patients with chronic myeloid leukemia. The plasma was deproteinated by methanol containing d8-IM. The separation was achieved on a 1.7 mým C18 column with a linear gradient (4 mM NH4COOH and AcCN; pH 3.2). FIA was performed at flow rate of 0.03 mL/min (0.1% formic acid in methanol). MRM mode on the tandem mass spectrometer in positive ESI were used for detection.The total analysis times were 3.2 (LC) and 0.75 min (FIA). Both methods were successfully validated and applied to the patients samples. The limits of quantification were 4.1 and 30.8 ng/mL; imprecisions were less than 5.7% and recovery ranged between 93 and 105%, for the LC and FIA, respectively. The methods correlated with a mean difference of 1.46 ng/mL (SD 28.95 ng/mL). Both methods are suitable for the therapeutic drug monitoring of IM in plasma and can be used in routine clinical
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/NS9627" target="_blank" >NS9627: The role of cellular transport mechanisms in efficacy of imatinib mesylate treatment in patients with chronic myeloid leukemia</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinica Chimica Acta
ISSN
0009-8981
e-ISSN
—
Volume of the periodical
411
Issue of the periodical within the volume
23-24
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
—
UT code for WoS article
000283916600018
EID of the result in the Scopus database
—