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Flow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10215584" target="_blank" >RIV/61989592:15110/10:10215584 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Flow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma

  • Original language description

    The aim of this study was to develop, validate and compare FIA- and UHPLC-MS/MS methods for the determination of imatinib (IM) in plasma from patients with chronic myeloid leukemia. The plasma was deproteinated by methanol containing d8-IM. The separation was achieved on a 1.7 mým C18 column with a linear gradient (4 mM NH4COOH and AcCN; pH 3.2). FIA was performed at flow rate of 0.03 mL/min (0.1% formic acid in methanol). MRM mode on the tandem mass spectrometer in positive ESI were used for detection.The total analysis times were 3.2 (LC) and 0.75 min (FIA). Both methods were successfully validated and applied to the patients samples. The limits of quantification were 4.1 and 30.8 ng/mL; imprecisions were less than 5.7% and recovery ranged between 93 and 105%, for the LC and FIA, respectively. The methods correlated with a mean difference of 1.46 ng/mL (SD 28.95 ng/mL). Both methods are suitable for the therapeutic drug monitoring of IM in plasma and can be used in routine clinical

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NS9627" target="_blank" >NS9627: The role of cellular transport mechanisms in efficacy of imatinib mesylate treatment in patients with chronic myeloid leukemia</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clinica Chimica Acta

  • ISSN

    0009-8981

  • e-ISSN

  • Volume of the periodical

    411

  • Issue of the periodical within the volume

    23-24

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000283916600018

  • EID of the result in the Scopus database