Protopine and allocryptopine increase mRNA levels of cytochromes P450 1A in human hepatocytes and HepG2 cells independently of AhR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10222122" target="_blank" >RIV/61989592:15110/11:10222122 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Protopine and allocryptopine increase mRNA levels of cytochromes P450 1A in human hepatocytes and HepG2 cells independently of AhR

Original language description

We examined whether alkaloids protopine or allocryptopine affect the expression of cytochromes P450 (CYPs) 1A1 and 1A2 in human hepatocytes and HepG2 cells. In HepG2 cells, protopine and allocryptopine increased CYP1A1 mRNA levels at concentrations from25 and 10 ?M, respectively. Both protopine and allocryptopine also increased CYP1A1 and CYP1A2 mRNA levels in human hepatocytes. Using gene reporter assays performed in transiently transfected HepG2 cells, we demonstrated that the induction of CYP1A1 expression by either protopine or allocryptopine was associated with negligible activation of aryl hydrocarbon receptor. CYP1A mRNA levels induced by protopine or allocryptopine in both HepG2 cells and human hepatocytes did not result in elevated CYP1A protein or activity levels. We conclude that the use of products containing protopine or allocryptopine may be considered safe in terms of possible induction of CYP1A enzymes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Volume of the periodical

203

Issue of the periodical within the volume

2

Country of publishing house

IE - IRELAND

Number of pages

7

Pages from-to

135-141

UT code for WoS article

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EID of the result in the Scopus database

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