Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33140851" target="_blank" >RIV/61989592:15110/12:33140851 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/12:00387882
Result on the web
<a href="http://dx.doi.org/10.1038/cdd.2011.150" target="_blank" >http://dx.doi.org/10.1038/cdd.2011.150</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/cdd.2011.150" target="_blank" >10.1038/cdd.2011.150</a>
Alternative languages
Result language
angličtina
Original language name
Nucleoporin NUP153 guards genome integrity by promoting nuclear import of 53BP1
Original language description
53BP1 is a mediator of DNA damage response (DDR) and a tumor suppressor whose accumulation on damaged chromatin promotes DNA repair and enhances DDR signaling. Using foci formation of 53BP1 as a readout in two human cell lines, we performed an siRNA-based functional high-content microscopy screen for modulators of cellular response to ionizing radiation (IR). Here, we provide the complete results of this screen as an information resource, and validate and functionally characterize one of the identified'hits': a nuclear pore component NUP153 as a novel factor specifically required for 53BP1 nuclear import. Using a range of cell and molecular biology approaches including live-cell imaging, we show that knockdown of NUP153 prevents 53BP1, but not severalother DDR factors, from entering the nuclei in the newly forming daughter cells. This translates into decreased IR-induced 53BP1 focus formation, delayed DNA repair and impaired cell survival after IR. In addition, NUP153 depletion exace
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CELL DEATH AND DIFFERENTIATION
ISSN
1350-9047
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
798-807
UT code for WoS article
000302784400008
EID of the result in the Scopus database
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