Preparation of conjugates of cytotoxic lupane triterpenes with biotin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33156196" target="_blank" >RIV/61989592:15110/15:33156196 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/15:33156196
Result on the web
<a href="http://pubs.acs.org/doi/ipdf/10.1021/acs.bioconjchem.5b00567" target="_blank" >http://pubs.acs.org/doi/ipdf/10.1021/acs.bioconjchem.5b00567</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.bioconjchem.5b00567" target="_blank" >10.1021/acs.bioconjchem.5b00567</a>
Alternative languages
Result language
angličtina
Original language name
Preparation of conjugates of cytotoxic lupane triterpenes with biotin
Original language description
To better understand the mechanism of action of antitumor triterpenes, we are developing methods to identify their molecular targets. A promising method is based on combination of quantitative proteomics with SILAC and uses active compounds anchored to magnetic beads via biotin-streptavidin interaction. We developed a simple and fast solid-phase synthetic technique to connect terpenes to biotin through a linker. Betulinic acid was biotinylated from three different conjugation sites for use as a standard validation tool since many molecular targets of this triterpene are already known. Then, a set of four other cytotoxic triterpenoids was biotinylated. Biotinylated terpenes were similarly cytotoxic to their nonbiotinylated parents, which suggests that the target identification should not be influenced by linker or biotin. The developed solid-phase synthetic approach is the first attempt to use solid phase synthesis to connect active triterpenes to biotin and is applicable as a general procedure for routine conjugation of triterpenes with other molecules of choice.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioconjugate Chemistry
ISSN
1043-1802
e-ISSN
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Volume of the periodical
26
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
2563-2570
UT code for WoS article
000366877400030
EID of the result in the Scopus database
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