Imaging Flow Cytometry as a Sensitive Tool to Detect Low-Dose-Induced DNA Damage by Analyzing 53BP1 and gamma H2AX Foci in Human Lymphocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33158244" target="_blank" >RIV/61989592:15110/15:33158244 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/cyto.a.22731" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22731</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cyto.a.22731" target="_blank" >10.1002/cyto.a.22731</a>

Result language

angličtina

Original language name

Imaging Flow Cytometry as a Sensitive Tool to Detect Low-Dose-Induced DNA Damage by Analyzing 53BP1 and gamma H2AX Foci in Human Lymphocytes

Original language description

Ionizing radiation induced foci (IRIF) are considered the most sensitive indicator for DNA double-strand break (DSB) detection. Monitoring DSB induction by low doses of ionizing radiation is important due to the increasing exposure in the general population. gamma H2AX and 53BP1 are commonly used molecular markers for in situ IRIF assessment. Imaging flow cytometry (IFC) via ImageStream system provides a new opportunity in this field. We analyzed the formation of 53BP1, gamma H2AX foci and their co-localization induced by gamma-rays (2, 5, 10, 50, 200 cGy) in human lymphocytes using ImageStream and the automated microscopic system Metafer. We observed very similar sensitivity of both systems for the detection of endogenous and low-dose-induced IRIF. Statistically significant induction of gamma H2AX foci was found at doses of 2 and 10 cGy using ImageStream and Metafer, respectively. Statistically significant induction of 53BP1 foci was evident at doses }= 5 cGy when analyzed by IFC. Ana

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytometry. Part A

ISSN

1552-4922

e-ISSN

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Volume of the periodical

87A

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1070-1078

UT code for WoS article

000367670800003

EID of the result in the Scopus database

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