Application of Molecular diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A73574810" target="_blank" >RIV/61989592:15110/15:73574810 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Application of Molecular diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Original language description

The infections caused by extended-spectrum -lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients&apos; clinical material, was developed. This study focused on bla(CTX-M) and bla(SHV) determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the bla(CTX-M). In two samples, the ESBL bla(SHV-ESBL) gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

AIMS ENVIRONMENTAL SCIENCE

ISSN

2372-0344

e-ISSN

—

Volume of the periodical

2015

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

1

UT code for WoS article

000363875600015

EID of the result in the Scopus database

—