Enantioseparation of D,L-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry-Fast and efficient tool for D- and L-2-hydroxyglutaracidurias diagnosis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33161400" target="_blank" >RIV/61989592:15110/16:33161400 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/16:33161400
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0021967316307191" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967316307191</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2016.05.095" target="_blank" >10.1016/j.chroma.2016.05.095</a>

Result language
angličtina
Original language name
Enantioseparation of D,L-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry-Fast and efficient tool for D- and L-2-hydroxyglutaracidurias diagnosis
Original language description
A novel capillary electrophoresis tandem mass spectrometry method for the enantioseparation and identification of 2-hydroxyglutaric acid enantiomers without derivatization for clinical purposes was described. Vancomycin chloride was used as an efficient chiral selector for the discrimination of 2-hydroxyglutaric acid enantiomers by capillary electrophoresis employed complete capillary filling method. The obtained resolution was 2.05. Hyphenation of CE with tandem mass spectrometry allows a reliable identification of separated enantiomers as well as their quantification. The method was validated and applied for the separation, identification and determination of 2-hydroxyglutaric enantiomers in urine samples obtained from healthy patients and two urine samples obtained from child patients suffering from high urine excretion of 2-hydroxyglutaric acid. Abnormal excretion of D-hydroxyglutaric acid was found in both child urine samples (104.5 +/- 2.1 and 2200.0 +/- 12.6 mmol/mol of creatinine, respectively). The limits of detection for D- and L-hydroxyglutaric acid were 31 and 38 nmol/L, respectively.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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