Reproducibility of Uniform Spheroid Formation in 384-Well Plates: The Effect of Medium Evaporation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33162813" target="_blank" >RIV/61989592:15110/16:33162813 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1177/1087057116651867" target="_blank" >http://dx.doi.org/10.1177/1087057116651867</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1087057116651867" target="_blank" >10.1177/1087057116651867</a>

Result language

angličtina

Original language name

Reproducibility of Uniform Spheroid Formation in 384-Well Plates: The Effect of Medium Evaporation

Original language description

Spheroid cultures of cancer cells reproduce the spatial dimension-induced in vivo tumor traits more effectively than the conventional two-dimensional cell cultures. With growing interest in spheroids for high-throughput screening (HTS) assays, there is an increasing demand for cost-effective miniaturization of reproducible spheroids in microtiter plates (MPs). However, well-to-well variability in spheroid size, shape, and growth is a frequently encountered problem with almost every culture method that has prevented the transfer of spheroids to the HTS platform. This variability partly arises due to increased susceptibility of MPs to edge effects and evaporation-induced changes in the growth of spheroids. In this study, we examined the effect of evaporation on the reproducibility of spheroids of tumor and nontumor cell lines in 384-well plates, and show that culture conditions that prevent evaporation-induced medium loss result in the formation of uniform spheroids across the plate. Additionally, we also present a few technical improvements to increase the scalability of the liquid-overlay spheroid culturing technique in MPs, together with a simple software routine for the quantification of spheroid size. We believe that these cost-effective improvements will aid in further improvement of spheroid cultures for HTS drug discovery.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomolecular Screening

ISSN

1087-0571

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

923-930

UT code for WoS article

000384469400005

EID of the result in the Scopus database

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