Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F17%3A73584125" target="_blank" >RIV/61989592:15110/17:73584125 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41467-017-01439-x" target="_blank" >https://www.nature.com/articles/s41467-017-01439-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-017-01439-x" target="_blank" >10.1038/s41467-017-01439-x</a>

Result language

angličtina

Original language name

Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia

Original language description

Maintenance of genome integrity via repair of DNA damage is a key biological process required to suppress diseases, including Fanconi anemia (FA). We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a component of the FA core complex, and used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (genetic suppressor) interactions for FA. Here we show that loss of the BLM helicase complex suppresses FANCC phenotypes and we confirm this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that function as part of the FA I-D2 complex, indicating that this interaction is not limited to the FA core complex, hence demonstrating that systematic genome-wide screening approaches can be used to reveal genetic viable interactions for DNA repair defects.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Communications

ISSN

2041-1723

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

1238

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

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UT code for WoS article

000414229000002

EID of the result in the Scopus database

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