Nanodiamonds of different sizes and charges show different ability to stimulate and mature murine dendritic cells in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591336" target="_blank" >RIV/61989592:15110/18:73591336 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nanodiamonds of different sizes and charges show different ability to stimulate and mature murine dendritic cells in vitro

Original language description

Nanodiamonds represent nanoparticles with various properties depending on their size, charge, shape, surface modification atc. They were modified to exhibit very stable fluorescence that could help by tracking of desired molecules coupled to the nanodiamonds. Nanodiamonds of different sizes with different charges were tested in this analysis. Particles ranging from 5 to 80 nm with a positive or negative surface charge were examined for their immunostimulatory properties. Murine bone marrow-derived dendriticcells were pulsed for 24 hours with these construct and different maturation marker were determined by flow cytometry including CD11c, CD80, CD86, CD40 as well as MHCI and MHCII molecules. Our data show that all the types of nanodiamonds stimulate BMDCs very slightly in comparison to lipopolysaccharide (LPS). Only moderate increase in CD86 and CD80 maturation markers was observed. On the other hand it is interesting that all the sizes of diamonds stimulated expression of MHCI molecules comparably to LPS and limited expression of MHCII molecules when compared to LPS or not pulsed control. After analyzing the effect of nanodiamonds surface charge on pulsed BMDC response, generally the positive charge is more effective in BMDCs stimulation (CD86 and CD80). In contrast the negative charge increases significantly the MHCI expression of dendritic cells. Finally, all types of nanodiamonds-or LPS-stimulated BMDCs and control cells (not pulsed BMDCs) were analyzed using the light microscopy. The nanodiamonds of sizes between 40 and 80 nm seemed to be toxicfor the BMDCs, as those exposed to nanodiamonds have had almostno dendrites and many of them have been destroyed especially when the 80 nm size of nanodiamond was used. Here both immunomodulatory and toxic effects of nanodiamonds were reported dependent on the nature of analyzed nanodiamonds. This needs to be considered in opting their application in biologic systems including humans.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

11th International Conference Drug Delivery Systems Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants, Modern Drug Delivery Systems and Cell Therapy

ISBN

978-80-907074-6-7

ISSN

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e-ISSN

neuvedeno

Number of pages

12

Pages from-to

59-70

Publisher name

nakladatelství Machovský

Place of publication

Olomouc

Event location

Telč

Event date

Jun 5, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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