A method of determining the amount of DNA in sample
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594376" target="_blank" >RIV/61989592:15110/19:73594376 - isvavai.cz</a>
Result on the web
<a href="https://worldwide.espacenet.com/patent/search/family/062791482/publication/EP3395956A1?q=EP%203395956" target="_blank" >https://worldwide.espacenet.com/patent/search/family/062791482/publication/EP3395956A1?q=EP%203395956</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A method of determining the amount of DNA in sample
Original language description
The invention concerns a method of determining the amount of DNA in samples, comprising the following steps: (i) a sample is incubated in a solution of at least one fluorescent substance selected from the group consisted of DAPI, Hoechst 33258, Hoechst 33342 or Hoechst 34580, which, in the course of this incubation, binds to the DNA present in the sample; (ii) the sample is washed with a washing solution and/or water, which washed away the fluorescent substances not bound to the DNA in the sample; (iii) the fluorescent substances bound to the DNA in the sample are eluted from the DNA with an aqueous elution solution of at least one substance selected from the group consisted of SDS, LiDS and sarcosyl, wherein the concentration of SDS, LiDS and sarcosyl is at least 0.1 % (w/v); (iv) the amount of DNA is determined in the elution solution from the previous step by measuring the fluorescent signal of the fluorescent substance used in step (i). The invention further relates to a method of determining the amount of EdU and/or BrdU and/or CldU and/or IdU incorporated into DNA and/or the content of proteins and/or the amount of RNA and/or the amount of specific DNA sequences and/or the amount of sugars and/or the amount of lipids and/or the amount of fluorescently-labelled DNA and/or fluorescently-labelled RNA and/or fluorescently-labelled proteins in cells and tissues; to a method of determining the replication activity in cells and tissues.
Czech name
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Czech description
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Classification
Type
P - Patent
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data specific for result type
Patent/design ID
3395956
Publisher
EPO_1 -
Publisher name
European Patent Office
Place of publication
Munich, The Hague, Berlin, Vienna, Brussels
Publication country
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Date of acceptance
Nov 13, 2019
Owner name
Univerzita Palackého v Olomouci
Method of use
A - Výsledek využívá pouze poskytovatel
Usage type
A - K využití výsledku jiným subjektem je vždy nutné nabytí licence