Anti-domain 1 β2 Glycoprotein Antibodies Increase Expression of Tissue Factor on Monocytes and Activate NK Cells and CD8+ Cells in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73599273" target="_blank" >RIV/61989592:15110/20:73599273 - isvavai.cz</a>

Alternative codes found

RIV/00098892:_____/20:N0000040

Result on the web

<a href="https://autoimmunhighlights.biomedcentral.com/articles/10.1186/s13317-020-00128-y" target="_blank" >https://autoimmunhighlights.biomedcentral.com/articles/10.1186/s13317-020-00128-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13317-020-00128-y" target="_blank" >10.1186/s13317-020-00128-y</a>

Result language

angličtina

Original language name

Anti-domain 1 β2 Glycoprotein Antibodies Increase Expression of Tissue Factor on Monocytes and Activate NK Cells and CD8+ Cells in vitro

Original language description

Background:β2-Glycoprotein I (β2GPI) represents the major antigenic target for antiphospholipid antibodies (aPL), with domain 1 (D1) being identified as a risk factor for thrombosis and pregnancy complications in APS. We aimed to analyse the ability of aPL, and particularly anti-D1 β2GPI, to stimulate prothrombotic and proinflammatory activity of immune cells in vitro. Methods:Peripheral blood mononuclear cells (PBMCs) from 11 healthy individuals were incubated with: (1) “anti-D1(+)”—pooled plasma derived from patients suspected of having APS contained anticardiolipin antibodies (aCL), lupus anticoagulant (LA), anti-β2GPI and anti-D1 β2GPI; (2) “anti-D1(−)”—pooled plasma from patients suspected of having APS contained aCL, LA, anti-β2GPI, and negative for anti-D1 β2GPI; (3) “seronegative”—negative for aPL. Results:The presence of anti-D1(+) and anti-D1(−) plasma resulted in increased HLA-DR and CD11b on monocytes. While only anti-D1(+) plasma markedly increased the percentage and median fluorescence intensity (MFI) of CD142 (tissue factor, TF) on monocytes in comparison with those cultured with anti-D1(−) and seronegative plasma. Anti-D1(+) plasma resulted in increased percentage and MFI of activation marker CD69 on NK and T cytotoxic cells. Expression of IgG receptor FcγRIII(CD16) on monocytes and NK cells was down-regulated by the anti-D1(+) plasma. Conclusions:Taking together, our study shows the ability of patient-derived aPL to induce immune cell activation and TF expression on monocytes. For the first time, we demonstrated the influence of anti-D1 β2GPI on the activation status of monocytes, NK and cytotoxic T cells. Our findings further support a crucial role of D1 epitope in the promotion of thrombosis and obstetrical complications in APS.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Autoimmunity Highlights

ISSN

2038-0305

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

1

Country of publishing house

IT - ITALY

Number of pages

9

Pages from-to

"’5(1)’"-"’5(9)’"

UT code for WoS article

000519448900001

EID of the result in the Scopus database

2-s2.0-85082653626