Reactive Oxygen Species Imaging in U937 Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73604923" target="_blank" >RIV/61989592:15110/20:73604923 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73604923
Result on the web
<a href="http://www.frontiersin.org/articles/10.3389/fphys.2020.552569/full" target="_blank" >http://www.frontiersin.org/articles/10.3389/fphys.2020.552569/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2020.552569" target="_blank" >10.3389/fphys.2020.552569</a>
Alternative languages
Result language
angličtina
Original language name
Reactive Oxygen Species Imaging in U937 Cells
Original language description
The U937 cell culture is a pro-monocytic, human histiocytic lymphoma cell line. These monocytes can differentiate into either macrophages or dendritic cells (antigenpresenting cells) depending on the initiators. The U937 cells activated in the presence of phorbol 12-myristate 13-acetate (PMA) change their morphology into macrophagelike cells creating pseudopodia and adhering generously. Macrophages are known to produce reactive oxygen species (ROS) mostly during phagocytosis of foreign particles, an important non-specific immune response. Recently, we have focused on the role of hydroxyl radical (HO_) and provide evidence on its importance for differentiation in U937 cells. Based on electron paramagnetic resonance (EPR) spectroscopy combined with confocal laser scanning microscopy (CLSM), formation of HO_ was confirmed within the cells undergoing differentiation and/or apoptosis during the PMA treatment. This study aims to increase our knowledge of ROS metabolism in model cell lines used in human research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Physiology
ISSN
1664-042X
e-ISSN
—
Volume of the periodical
11
Issue of the periodical within the volume
OCT
Country of publishing house
CH - SWITZERLAND
Number of pages
7
Pages from-to
"552569-1"-"552569-7"
UT code for WoS article
000584817400001
EID of the result in the Scopus database
2-s2.0-85094626827